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pet22b pa his  (Addgene inc)


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  • 91

    Structured Review

    Addgene inc pet22b pa his
    Pet22b Pa His, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet22b+pa+his/pET22b+-+PA+(Plasmid+%2384863)/pm36604500-135-10-13
    Average 91 stars, based on 4 article reviews
    pet22b pa his - by Bioz Stars, 2026-10
    91/100 stars

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    Modification:

    Article Title: Mechanism of NAIP—NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5
    Article Snippet: .. Plasmids pET15b-LFn-Fla (Addgene no. 84871), pET15-LFn-FlaAAA (Addgene no. 84872) and pET22b-PA-His (modified from Addgene no. 84863) were gifted by R. Vance 3 . .. Recombinant expression of NAIP5 was carried out by either transiently transfecting Expi293F cells (Thermo Fisher no. A14635) grown in suspension culture using polyethylenimine (PEI) (Polysciences no. 23966) reagent, or directly purified from a homemade HEK293F cell line stably expressing NAIP5 protein.

    Article Title: Mechanism of NAIP-NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5.
    Article Snippet: The nucleotide-binding domain (NBD), leucine rich repeat (LRR) domain containing protein family (NLR family) apoptosis inhibitory proteins (NAIPs) are cytosolic receptors that play critical roles in the host defense against bacterial infection.. NAIPs interact with conserved bacterial ligands and activate the NLR family caspase recruitment domain containing protein 4 (NLRC4) to initiate the NAIP—NLRC4 inflammasome pathway.. Here we found the process of NAIP activation is completely different from NLRC4.



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    91
    Addgene inc pet22b pa his
    Pet22b Pa His, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet22b+pa+his/pET22b+-+PA+(Plasmid+%2384863)/pm36604500-135-10-13
    Average 91 stars, based on 1 article reviews
    pet22b pa his - by Bioz Stars, 2026-10
    91/100 stars
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